Journal: Frontiers in Endocrinology
Article Title: Steroidogenic differentiation of human amniotic membrane-derived mesenchymal stem cells into a progesterone-/androgen-producing cell lineage by SF-1 and an estrogen-producing cell lineage by WT1−KTS
doi: 10.3389/fendo.2024.1410433
Figure Lengend Snippet: Differentiation capacity of hAmMSCs. (A) Adipogenic differentiation was evidenced by Oil Red O staining. Lipid vacuoles were formed in the hAmMSCs. (B) Osteogenic differentiation was evidenced by Alizarin Red S staining. Mineralized matrix was formed in the hAmMSCs. (C) The hAmMSCs cultured with chondrogenic differentiation medium formed pellets. Note that the cells did not form pellets in the control medium (RPMI 1640 plus 10% Fetal Calf Serum). Chondrogenic differentiation was evidenced by Alcian blue staining. Scale bar, 100 µm. hAmMSCs, human amniotic membrane-derived mesenchymal stem cells.
Article Snippet: To induce adipogenic differentiation, the amniotic membrane-derived cells were seeded in Mesenchymal Stem Cell Adipogenic Differentiation Medium (PromoCell) at a density of 2×10 4 cells/cm 2 and cultured for 4 weeks.
Techniques: Staining, Cell Culture, Control, Membrane, Derivative Assay